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Old 12-13-2017, 10:21 PM   #4
sbarberan
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Location: Santa Cruz, CA

Join Date: Feb 2017
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How many cycles of PCR did you perform? We have seen a big peak ~280 when you over amplify libraries.

I think that these products at 280 could be 'bulge' products generated when you deplete primers, see (http://www.lsi.umich.edu/files/SmallRNACloning.pdf) and search for bulge.
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