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fbarreto · 12-14-2017, 05:22 PM

Hi, sbarberan,

We did 14 PCR cycles, with total starting RNA of ~700 ng. In consultation with our local sequencing core who does small RNA preps, they suggest these look fine, and we're proceeding with PAGE size-selection of the 140-160 bp bands. Fingers crossed.

I will update the thread when I get new BioA traces or sequences.

Thanks!

12-14-2017, 05:22 PM	#5
fbarreto Member Location: San Diego Join Date: Jan 2010 Posts: 15	Hi, sbarberan, We did 14 PCR cycles, with total starting RNA of ~700 ng. In consultation with our local sequencing core who does small RNA preps, they suggest these look fine, and we're proceeding with PAGE size-selection of the 140-160 bp bands. Fingers crossed. I will update the thread when I get new BioA traces or sequences. Thanks!