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Old 12-14-2017, 05:22 PM   #5
fbarreto
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Location: San Diego

Join Date: Jan 2010
Posts: 15
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Hi, sbarberan,

We did 14 PCR cycles, with total starting RNA of ~700 ng. In consultation with our local sequencing core who does small RNA preps, they suggest these look fine, and we're proceeding with PAGE size-selection of the 140-160 bp bands. Fingers crossed.

I will update the thread when I get new BioA traces or sequences.

Thanks!
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