Hi,
I am a newbie in the field of RNA-Seq and i apologize if this query seems too trivial. I have Solid data in fasta format and i am supposed to remove the P2 adapter.
The P2 adapter sequence is:
5' ------- (-) AGAGAATGAGGAACCCGGGG CAGTT--- - 3'
3' ------- (-) TCTCTTACTCCTTGGGCCCC GTC----- - 5'
Does this mean that the
5' ------(-) AGAGAATGAGGAACCCGGGG CAGTT--- -3' will be present at the 3'end of the reads while the
3' ------- (-) TCTCTTACTCCTTGGGCCCC GTC----- - 5' would be present at the 5'end of the read?
Thanks,
vini
I am a newbie in the field of RNA-Seq and i apologize if this query seems too trivial. I have Solid data in fasta format and i am supposed to remove the P2 adapter.
The P2 adapter sequence is:
5' ------- (-) AGAGAATGAGGAACCCGGGG CAGTT--- - 3'
3' ------- (-) TCTCTTACTCCTTGGGCCCC GTC----- - 5'
Does this mean that the
5' ------(-) AGAGAATGAGGAACCCGGGG CAGTT--- -3' will be present at the 3'end of the reads while the
3' ------- (-) TCTCTTACTCCTTGGGCCCC GTC----- - 5' would be present at the 5'end of the read?
Thanks,
vini
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