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Old 06-20-2013, 06:04 AM   #2
Brunda
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Location: Brno, Czech Rep.

Join Date: Jan 2013
Posts: 10
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Hello Carly, how did you resolve your problem? I just stumbled onto thread on http://seqanswers.com/forums/showthread.php?t=14511

i wonder if it's possible to do the qubit measurement instead of QPCR (to be sure you have enough sample) and then going for the norm kit.

the advantage of QPCR is that it "counts" only dsDNA fragments with both adaptors ligated, so you can be sure it is specific.
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