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Old 07-19-2013, 05:21 AM   #2
cliffbeall
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Location: Ohio

Join Date: Jan 2010
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RNA will not run on a gel exactly according to its size because it is single stranded and the secondary structure can be variable. To get an accurate size you need to use a denaturing gel (formaldehyde, urea, methyl mercury) and compare it to RNA standards.

Since you see 2 pretty sharp bands, your RNA is probably fine.

Edit: There is also a bioanalyzer chip for RNA that would be an option if you have access to an instrument.

Last edited by cliffbeall; 07-19-2013 at 05:24 AM.
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