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Old 08-19-2014, 03:46 PM   #5
Mamta
Junior Member
 
Location: US

Join Date: Sep 2012
Posts: 2
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HI all,

Something similar but not exactly the same.
I am having issues with Illumina trueseq custom amplicon data. First Fastqc shows 93% duplication level. Is that a big concern. I have small portion of the genome sequenced
2. There is around 1.3% of unmapped overrepresented reads..Are these adapters. Iam nt sure if its a good idea to trim them.

Lastly I have several regions with only Forward reads and no reverse reads at all... What could be the possible reasons for this?

I would really appreciate if someone could help or direct me to any document/paper that might help.

Thanks a lot!
'Mamta
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