Thread: miRNA Discovery
View Single Post
Old 08-20-2014, 11:30 AM   #1
rndouglas
Member
 
Location: USA

Join Date: Jun 2012
Posts: 23
Default miRNA Discovery

I may be a bit naive, but I'm trying to see if I can identify miRNA-precursors in a new genome assembly I recently made.

I have available to me:
  • de novo assembled genome
  • 1x100bp Illumina total RNA RNAseq
  • Illumina sRNA RNAseq

Is there any program that can whittle down potential miRNA-precursor loci using all three sets of sequences rather than going through everything by hand?

I suppose in my head I see something like:
map 100bp read to genome --> look for sRNAs that bind within the mapped 100bp potential precursor --> analyze the region to see if it looks decent to actually be a miRNA precursor --> add it to my list to go back and verify experimentally someday.
rndouglas is offline   Reply With Quote