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Old 08-31-2015, 12:59 AM   #1
Jane M
Senior Member
 
Location: Paris

Join Date: Aug 2011
Posts: 239
Question Single Cell analysis for DNA-Seq

Hello everybody,

I will get targeted single cell DNA data in the upcoming days. I have never analysed single cell data, so I searched on this forum and on internet for information, but I haven't been able to find a lot of things for DNA. My goal will be to identify short mutations (already identified by WES and check using PGM).

My question is the following: can we analyse this type of data as it is commonly done for WES, WGS and targeted data (obtained from PGM or MiSeq instruments - without removal of PCR duplicates)?

I would also appreciate other information, like typical alignment rate or average quality,...

Thank you in advance for your help,
Jane
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