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Old 10-22-2008, 05:32 PM   #3
Junior Member
Location: Asian

Join Date: Feb 2008
Posts: 6

Thans ECO.
Actually I fragment our sample following the protocol from SOLiD, and no glycerol has been used. So we can measure the concentraion before clean up.
I clean up our sample using Qiagen Qiaquick pcr purification kit, and the concentration before and after clean up is 70ng/ul and 20ng/ul (nanodrop), respectively. The concentration before clean up is great different as I descripted yesterday, but after clean up it is almost the same from nanodrop and Agilent 2100.
I also believe that the bioanalyzer should more accurate than nanodrop, but from the 2%agarose gel image the concentration of fragment DNA is more close to nanodrop????

Last edited by floor121; 08-16-2011 at 10:41 AM.
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