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Old 08-11-2010, 10:03 AM   #2
Lee Sam
Location: Ann Arbor, MI

Join Date: Oct 2008
Posts: 57

Have you done verification against dbSNP? Have you filtered down your candidates to just those within known exons after alignment (often times PE reads "splash over" into intronic regions where variation is likely more liberally tolerated)?

EDIT: I'm actually really curious to hear about how many reads and read length and how many lanes you ran the sample on. We just got our HiSeq2k installed last week and we're running our first samples though it. Details would be fantastic.

Last edited by Lee Sam; 08-11-2010 at 12:29 PM.
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