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Old 05-17-2011, 08:45 AM   #7
aleferna
Senior Member
 
Location: sweden

Join Date: Sep 2009
Posts: 121
Default MiSeq vs IonTorrent

So I attended an Illumina conference, 2 weeks ago and of course I asked the obvious question... how is MiSeq better than PGM?
The answer was not as clear/black and white as I wanted, they said
1. PGM has lower quality, lots of Q20 bases??
2. Unproven technology
3. No Paired End sequencing

Well, other except for the PE, the other answers were kind of ambiguous. I'm trying to compile a comparison between the 2 devices as we are probably going to buy one of these machines next year. It would be nice to have the following raster:
1. Throughput/Hands On Time/Running Time per run
2. Quality of base 25, 50, 75, 100
3. Cost:
Equipment (PGM $50k, MiSeq $150k??)
Cost per run?
Cost per sample prep? (they said $750 for the MiSeq sequencing run, but didn't mention the sample prep.
4. Read length ?
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