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Old 06-15-2011, 04:53 PM   #14
krobison
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Location: Boston area

Join Date: Nov 2007
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Nextera cannot be directly used with PGM. With PGM's long read lengths, it would certainly be possible to design a "cheater" primer set to convert a Nextera library to a PGM library, but you would read through the Nextera primer on each read (and thereby waste some read length).

Also, at this time PGM libraries cannot have inserts longer than 150 nt; if your Nextera library is bigger than that it wouldn't currently be convertable.
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