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Old 12-04-2012, 08:17 AM   #1
Location: Spain

Join Date: Jan 2012
Posts: 10
Default Size selection: agarose gel / acrylamide gel

Hello everybody,

Maybe I am going to ask something very basic, but I haven't found the answer on the internet (probably I did the wrong queries)...

Could anyone tell me why we usually make size selection in agarose gels for the majority of libraries, but the small RNA libraries have to be size selected on acrylamide gels? I thought it was a matter of size, but I've also seen acrylamide gel size selection when doing directional mRNA seq using the small RNA seq protocol from illumina.

Is there any particular reason for which one should use one type of gels or the others?

Thanks a lot in advance, and sorry if the question is a bit stupid.

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