View Single Post
Old 02-07-2013, 11:08 PM   #5
razibus
Member
 
Location: France

Join Date: May 2011
Posts: 25
Default

I tried sonicating at 5kb and 3kb. The sonication profile looks good on the bioanalyzer.

However, the amount of DNA before sonication and after sonication/purification is very different. Indeed, the recovery ratio is not very good.

I'm measuring the DNA concentration with Qubit.

Do you, guys, have the same issue?
razibus is offline   Reply With Quote