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Old 02-11-2013, 04:34 AM   #7
razibus
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Location: France

Join Date: May 2011
Posts: 25
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Thank you for your answer, it's very relevant.

I tried Qiagen columns but also Zymo Research columns. Both works perfectly on PCR amplicons but poorly on sonicated gDNA.

I will give a try to Ampure XP. Nevertheless, the elution volume is ~30 and 40Ál, and I need only 10Ál. (I don't like SpeedVac very much)

Anyway, I don't get why we lost so much gDNA in purification columns...

Last edited by razibus; 02-11-2013 at 04:47 AM. Reason: Forgot a sentence
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