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Old 02-13-2013, 05:06 AM   #10
Location: Cambridge

Join Date: Nov 2012
Posts: 21

we use the dsDNA Qubit kit (or Picogreen) to quantitate before and after Covaris shearing. We believe that this is the most accurate reading as previously compared to the nanodrop 1000 & Bioanalyzer. qPCR is accurate, but takes a lot more time to do than Qubit. In our experience both the nanodrop 1000 and the 2100 overestimate DNA concentrations.
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