View Single Post
Old 06-27-2017, 12:02 PM   #6
Brian Bushnell
Super Moderator
 
Location: Walnut Creek, CA

Join Date: Jan 2014
Posts: 2,707
Default

Quote:
Originally Posted by WhatsOEver View Post
My information on the sequel so far:
1) Accuracy of >99.999%
Is this a consensus acc from assembly/mapping? As this would be coverage dependent, it is meaningless for me... What about individual read (CCS) accuracy? I know there is something mentioned on the PB website, but I don't really understand their graph http://www.pacb.com/smrt-science/smr...cing/accuracy/
The graph indicates that the more coverage you have, the higher the quality of the self-corrected data (meaning reads corrected by other reads in the same library, not reads corrected by themselves). Last I saw, our RSII was producing 86-87% accurate reads and Sequel was producing 80-83% accurate reads, according to PacBio's software. CCS accuracy simply depends on how long the movie is compared to the insert size; for long insert libraries that you would generate for SV's, you would not yield many useful multi-pass CCS reads.
Brian Bushnell is offline   Reply With Quote