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Old 08-27-2012, 04:52 PM   #1
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Location: USA

Join Date: Jul 2012
Posts: 184
Default qPCR on Nextera XT Libraries

Has anyone had any luck trying to quantify Nextera XT libraries using qPCR? I have tried it twice with no success. I have been able to quantify TruSeq libraries and they have been working just right. It looks like primer sequences are correct, so I don't know what could be causing the problem.
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