Tweet
Does anyone know the composition of the Tagment Wash Buffer in the Nextera DNA Flex Library Prep (20018704) protocol? Does it contain alcohol? PEG?
TIA
TIA
-
-
I would check out the SMART-SEQ2 protocol. They did homebrew something similar. I vaguely remember SDS to denature the enzyme. -
Hi BexMultiplex,
According to the Nextera Flex patent, TWB is:
-100 mM Tris HCl pH 8.5
-100 mM NaCL
-0.1% Tween 20
We have a Tecan, and are going to be reducing RXN volumes down to 1/4, and at that point TWB becomes a limiting reagent. I will be trying my own eventually, but I have not used it yet personally myself, so i can't promise it will work
If you are ever trying to find what a reagent is, looking at the patent (if you can find it) is probably the best way to get this kind of information. It also really helpful for understanding how a kit work when the protocol is vague.
Hope this helps!Comment
-
Thanks @UCan'tBcereus, that's really helpful!Comment
-
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
Comment