Hello,
are there any Illumina sequencing read primers that differ in thier last 13 bp?
Would
Read1 (from TruSeq):
ACACTCTTTCCCTACACGACGCTCTTCCGATCT
Read 2 (from Nextera Transposase Adapters):
TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG
work?
I am using a two step PCR (without ligation) to make the library, so I am really flexible in what read primers I can use.
PCR1: universal_primer+target_specific_primer
PCR2: Illumina_adapter+index+pad+universal_primer
read primer: pad+universal_primer
are there any Illumina sequencing read primers that differ in thier last 13 bp?
Would
Read1 (from TruSeq):
ACACTCTTTCCCTACACGACGCTCTTCCGATCT
Read 2 (from Nextera Transposase Adapters):
TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG
work?
I am using a two step PCR (without ligation) to make the library, so I am really flexible in what read primers I can use.
PCR1: universal_primer+target_specific_primer
PCR2: Illumina_adapter+index+pad+universal_primer
read primer: pad+universal_primer