View Single Post
Old 09-07-2017, 07:15 AM   #13
Junior Member
Location: Paris

Join Date: Sep 2017
Posts: 5

I will most likely substitute the quality scores, but how are they written in PacBio data.
But it is coded scores in fastq so 30 corresponds to something like >
This should work. It will mean canu is unable to trim reads and increase the real quality but I will also run it against illumina data to correct errors afterwards.

Thank you all

UPDATE: With substitution of a qscore of 30 for each base canu assembly has issues and produces a large number of contigs ~130. Possibly has issues in ranking overlap probabilities.

Last edited by anotherSAM; 09-11-2017 at 02:20 AM.
anotherSAM is offline   Reply With Quote