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Old 08-26-2015, 11:58 AM   #24
shamimbd7
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Location: saint louis, MO

Join Date: Apr 2015
Posts: 3
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Hi all,

I am trying to do ATAC-Seq experiment using plant samples. I am wondering whether there is any size selection step after PCR amplification ( Using ampure beads). If yes, what size I should select. OR Instead of size selection, should I just clean up my PCR products using Qiagen Mini Elute.

My second question is, whether the fixation of nuclei with formaldehyde affects the library making even if I do de-crosslinking after the tagmentation reaction.

I would highly appreciate your response.
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