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Old 08-28-2015, 02:10 PM   #26
shamimbd7
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Location: saint louis, MO

Join Date: Apr 2015
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Quote:
Originally Posted by Wonghe View Post
Yes I get my atac to work on 70K freshly trypsinize cells using the original Greenleaf protocol. Depending on the cell type you are working on you might need to adjust the lysis buffer content to make sure the cells are lysed before adding the transposase.
Hi Wonghe,

I am very interested to see bioanalyzer trace of a successful ATAC-Seq experiment. If possible, would you please share your bioanalyzer trace. If not, would you please describe how was the pattern.
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