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Old 10-01-2015, 12:49 PM   #29
jenna2790
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Location: Berkeley, CA

Join Date: Sep 2015
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Quote:
Originally Posted by Wonghe View Post
Hi All, I've been trying to do ATAC-seq on nuclei isolated from frozen tissues with sucrose gradient. Although I am able to obtain a nucleosome-like pattern (see above attachment) but after sequencing I get many background peaks which are very noisy and the peaks at the TSS were not distinct. Anyone has any idea to improve the method and reduce the background peaks?
I am also using frozen tissues. Were you able to eliminate the noise?
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