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Old 10-06-2015, 06:40 AM   #30
shamimbd7
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Location: saint louis, MO

Join Date: Apr 2015
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Quote:
Originally Posted by annrose View Post
Hi all

I am trying to do ATAC-seq on fromaldehyde fixed cells. But dont have much sucess with it. Was wondering if any of you have tried it ......any suggestion will be of great help...
Hi Annrose, I have isolated formaldehyde fixed nuclei and thinking to do ATAC-Seq experiment on that. Would you please mention whether you are getting long fragments after tagmentation or any other issues? I am also wondering whether you have reverse crosslinked your samples before going for PCR. I would highly appreciate your response.
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