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Old 02-29-2016, 01:28 AM   #39
mantis
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Location: living in the lab in Europe

Join Date: Jan 2016
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Quote:
Originally Posted by Runuply View Post
Hi guys, I also used that protocol for ATAC-seq from frozen tissue, PCR amplification 5 cycles; and 7 cycles for qPCR(1 to 3rd of Max Fluorescent). It is a little hard for me to control the exact cell number, but after transposition and purification I will use 1ul to check by Qubit, which usually shows around 10ng/ul in 10l (this should be equal to 25k cells or less). And after I did PCR amplification (in total 5+7 cycles), the purified DNA will be about 50ng/ul in 20ul.
The Bio analyzer is attached.

Looks good Runuply: there seems to be a good nucleosome pattern on the gel picture. I think I would advice you to try to get rid of the primer peak at 65 before submitting the sample for sequencing.
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