Hi there!
Anyone working with Illumina Nextera DNA Flex for Enrichment?
We are experiencing some problems related to the hybridization or capture step. We don't know why, but after pooling the samples (12 samples in one pool, total 8 pools) and doing the capture, there is always some pools that don't perform correctly and gave very low yield and concentration.
We have performed 6 96-plates and in each of the processing of these plates, there is always this issue, and it seems to be at random.
We pool by mass, and we always quantify the samples with Qubit before pooling them, so, it has no relation with the hibridized amount. I mean, we hybridize the same total nanograms on each pool, but some of them work properly and others just don't...all the samples are fresh gDNA.
Any idea??
Thanks a lot in advance!!!
Anyone working with Illumina Nextera DNA Flex for Enrichment?
We are experiencing some problems related to the hybridization or capture step. We don't know why, but after pooling the samples (12 samples in one pool, total 8 pools) and doing the capture, there is always some pools that don't perform correctly and gave very low yield and concentration.
We have performed 6 96-plates and in each of the processing of these plates, there is always this issue, and it seems to be at random.
We pool by mass, and we always quantify the samples with Qubit before pooling them, so, it has no relation with the hibridized amount. I mean, we hybridize the same total nanograms on each pool, but some of them work properly and others just don't...all the samples are fresh gDNA.
Any idea??
Thanks a lot in advance!!!
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