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Old 09-27-2017, 03:09 PM   #4
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,187
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For amplicons such as 16S that has been prepped using the same protocol, pooling based on dsDNA specific reagents such as PicoGreen results in up to 3x variation in output which is similar to bead or plate based normalisation methods.
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