Hello all,
I would like to calculate TMM normalization for identifying DE genes. I know that edgeR does all the normalization when you run the code but I would like to calculate TMM for one or two genes to manually to better understand the statistic behind it. I found this formula to do so ( below) but I am not sure what the component, factor means?
(raw.counts)*10^7/(librarysize*factors)
librarysize=total number of mapped reads.
I would really appreciate any suggestions and help.
Thanks !
I would like to calculate TMM normalization for identifying DE genes. I know that edgeR does all the normalization when you run the code but I would like to calculate TMM for one or two genes to manually to better understand the statistic behind it. I found this formula to do so ( below) but I am not sure what the component, factor means?
(raw.counts)*10^7/(librarysize*factors)
librarysize=total number of mapped reads.
I would really appreciate any suggestions and help.
Thanks !
Comment