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Old 04-25-2017, 06:26 AM   #3
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Location: Germany

Join Date: Apr 2012
Posts: 215

1) bacteria often have a higher GC-content than eukaryots, so this might be a starting point
2) You could filter your dataset for the known symbionts (which genomes are available, at least acc to a 5s google research) and try an assembly with the rest (depending on what this Hiseq data is: WGS/RNA-Seq?)
3) If you could get a collection of genomes of known insect symbionts, try merging all these and map against this set
4) Contact the people belonging to the abstract "Hybrid De Novo Assembly of the Leafhopper Genome with SMRT Sequencing Method" I also found via google
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