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Old 10-18-2013, 03:34 AM   #1
nexgengirl
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Location: Maryland

Join Date: Apr 2010
Posts: 31
Default RNAseq Genes Different By File

Hi,

I have diff files from ~20 different RNA sequencing experiments. A common GTF file was utilized to make these diff files. The number and identity of the genes in the diff files is different in each file. Some genes overlap but some do not. My question is:

If a gene is not present in the diff file but was present in the GTF file does that mean it is not expressed?

Any clarification is greatly appreciated.
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