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Old 06-26-2015, 02:12 AM   #1
EVELE
Junior Member
 
Location: France

Join Date: Jun 2015
Posts: 9
Default DESEQ2: How to use dispersion estimated from one dataset in another dataset

Hi all,

I have recently started using DeSeq2 and I would like to know if it is possible to do something like this:

I have 3 time course experiments (1 control and 2 treated (A and B)) but I do not have biological replicates for B treated experiment.

So I calculated the dispersion for experiment A versus control and I wonder if there is a way to use the same dispersion for the comparison of B versus control.
How to do that ? Is it biologically, scientifically "legal" to do that ?

By the way, there is evidence that they have similar dispersion and I'd like to avoid using the "blind" method.

I would appreciate it to let me know your ideas about it.
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