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Old 10-26-2013, 12:53 PM   #1
Location: Brazil

Join Date: Oct 2013
Posts: 12
Default RNA-seq: concern about quantification of transcriptome

Hi everybody!

I'd like to generate and quantify whole transcriptomes by using RNA-seq in the near -- well, not so near -- future but I am completely newbie on this method.

My doubt concerns mainly the quantification capacity of RNA-seq: how can RNA-seq be used to quantify transcripts if a PCR amplification has to be done after the adapter ligation in the library preparation setp? How can this amplification step not impair the post-sequencing quantification of transcripts?

Is the amount of PCR-amplified products directly proportional to the initial amount of cDNAs in this case?

Thank you in advance!

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