SEQanswers - View Single Post - RNA-seq: concern about quantification of transcriptome

mlacencio · 10-27-2013, 05:15 PM

Hi Chipper! Thank you for your reply!

Actually my main concern is about the number of PCR cycles. It is known that the quantification of PCR products is unreliable if PCR reaches its saturation level. In general, how many cycles are allowed in the amplification step of a library prep for RNA-seq?

Thank you again!

10-27-2013, 05:15 PM	#3
mlacencio Member Location: Brazil Join Date: Oct 2013 Posts: 12	Hi Chipper! Thank you for your reply! Actually my main concern is about the number of PCR cycles. It is known that the quantification of PCR products is unreliable if PCR reaches its saturation level. In general, how many cycles are allowed in the amplification step of a library prep for RNA-seq? Thank you again!