SEQanswers - View Single Post - RNA-seq: concern about quantification of transcriptome

benjamir · 10-31-2013, 01:11 PM

Hi Mlacencio,
The Illumina TruSeq RNA-seq protocol calls for 15 cycle (total RNA 0.1 ug - 4 ug optimized).
A recent single-cell method, Quartz-Seq, performs 21 cycles of whole transcriptome amplification PCR prior to 10 - 12 cycles of library PCR.

10-31-2013, 01:11 PM	#4
benjamir Junior Member Location: Houston, TX Join Date: Nov 2012 Posts: 4	Hi Mlacencio, The Illumina TruSeq RNA-seq protocol calls for 15 cycle (total RNA 0.1 ug - 4 ug optimized). A recent single-cell method, Quartz-Seq, performs 21 cycles of whole transcriptome amplification PCR prior to 10 - 12 cycles of library PCR.