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Old 10-31-2013, 01:11 PM   #4
Junior Member
Location: Houston, TX

Join Date: Nov 2012
Posts: 4

Hi Mlacencio,
The Illumina TruSeq RNA-seq protocol calls for 15 cycle (total RNA 0.1 ug - 4 ug optimized).
A recent single-cell method, Quartz-Seq, performs 21 cycles of whole transcriptome amplification PCR prior to 10 - 12 cycles of library PCR.
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