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Old 12-12-2017, 09:23 AM   #2
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,297

Hi arctan,
You might want to take an aliquot of your sample, heat it to 70 oC for a few minutes to denature it's secondary structure, snap cool and then retest for the presence of genomic DNA.

Although the Qubit dsDNA High Sensitivity fluor detects primarily double stranded polynucleotides, I do not think it distinguishes between double-strand DNA and double-strand RNA. So any secondary structure in your RNA can read as DNA.

To the extent your RNA has long stretches of double stranded regions, the 70 oC treatment will not be sufficient to denature it.

Another alternative would be to take an aliquot of your RNA and treat it with a very effective RNAse, maybe if you can find a mixture that can degrade both ssRNA and dsRNA. Then check this aliquot for DNA.

pmiguel is offline   Reply With Quote