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Old 06-08-2018, 05:11 AM   #11
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,297

Thanks for posting your results.
I suspect that most of your dsDNA flourimetric signal comes from double-stranded RNA--ie, RNA folded into secondary structures. My guess is that the fluor used does not distinguish between dsDNA, dsRNA and RNA/DNA heteroduplex.

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