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Old 01-04-2018, 12:09 AM   #3
ericoml
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Location: campinas

Join Date: Oct 2017
Posts: 3
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Thanks.

This sequence indeed appears at the 3' end of my reads. I believe you thought it was at 5' but this is because I cut the remaining sequences. One example of the full lenght adapter dimer read:

GAAGNGCACACGTCTGAACTCCAGTCACGCAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGACTACACATAATGCGACACGATAGAAC

I was concerned that the sequence was artificially introduced in the experimental steps. Now I'm convinced that it's related to sequencing of the immobilized oligos.
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