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Old 05-06-2018, 07:28 AM   #257
Timer123
Junior Member
 
Location: Wuhan.China

Join Date: Dec 2016
Posts: 4
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Dear professor Simone:
I am very grateful for your reply so quickly. Thank you. Your advice will be a great way to check our reaction successful or not. And I will try it recently.
Before that I omitted an important message: our material is about a few hundred plant cells.
Previously, I got a good quality RNA-seq library (Ensure by data analysis), which also check the preamplification reaction by agarose gel electrophoresis, and as the picture shows, with bright bands stuck in the well. We still do not kown why this happens?
https://wx3.sinaimg.cn/mw690/c56dbb6...204c0cota5.jpg
And this time, I follow the protocol again(with the same material), a very lower ds cDNA field (4 times less) I get, it really make me crazy, do you have any suggestion?
Best
Student YangKe

Last edited by Timer123; 05-07-2018 at 01:55 AM.
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