View Single Post
Old 04-25-2017, 06:20 AM   #2
Location: Sweden

Join Date: Aug 2011
Posts: 30

Hi! You can ligate the BCA to any DNA and then do a barcoded PCR, e.g if you want to use genomic DNA or have amplicons without the ONT pcr-priming tail. We also tend to get lower than recommended yield of DNA after the final streptavidin bead purification, but I think 20 ng/ul is still high. If you only get 20 ng in total then something is likely wrong, perhaps adaptors are too old or your starting concentration was off.
Ola is offline   Reply With Quote