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Old 09-19-2012, 05:53 AM   #3
koadman
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Location: Sydney, Australia

Join Date: May 2010
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Thanks for the reply MrGuy, it hadn't occurred to me that we might be underloading some samples. The Nextera XT protocol had us tagment 1ng material per sample, then amplify that material with 12 cycles PCR before carrying out the normalization, so in principle there should have been much more than 1ng per sample going into normalization. However, the person making the libs reserved a portion of the PCR product so perhaps it's as simple as just reserving less next time.
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