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Old 08-26-2013, 05:12 AM   #1
juelraja
Junior Member
 
Location: Finalnd

Join Date: Aug 2013
Posts: 3
Default Agilent Bioanalyzer: Unexpected signal in 5S region

Hi guys!

I keep having this problem with zebrafish RNA isolated with Tri Reagent. On nanodrop everything seems to be OK (260/280 ratio ~2 and 260/230 ratio >2).

Do you have any idea what can cause this unexpected signal and how to get rid of it? Can I expect this to do harm later on with RT-qPCR?

Electropherogram seems quite nice but RIN values cannot be calculated.. (Hopefully managed to upload a picture)
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