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Old 09-30-2013, 03:52 AM   #3
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,315

Sometimes with Trizol samples you see enormous small RNA peaks that freak out the Agilent software. Nothing wrong with the samples though. They just produced a high yield of small RNA. In your case I don't see anything wrong with your chromatograms. That means the software produced an error that it should not have.

What we do is find the parameter setting (in the pane on the right side of the the 2100 Expert software window) that deals with the threshold for giving an "unexpected signal in the 5S region" and increase it until it removes the error.
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