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Old 01-10-2014, 01:24 PM   #1
fadss
Junior Member
 
Location: Britsh Columbia

Join Date: Jan 2014
Posts: 4
Smile Fragment analysis; microsatellite or SSR/STR

Has anyone been using next gen or preferably illumina for fragment analysis.
Right now my project are running multiplex for Sanger 3130xl machine to perform fragment size analysis.
But this limited to small sample and run 1 sample per capillary and require culture of bacteria for pure cullture.

I wish to know if illumina are limited to only sequencing?
or cant I just sequence the microsatellite loci and look at the length for fragment size of millions different strains and sort them using computer program some how.

So instead of having 10 to 100s sample size; I would have over 100,000 or millions.

but with multiplex(with many loci), how can i separate which strain to which since it all mix.


Thanks,
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