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Old 01-10-2014, 01:50 PM   #3
Junior Member
Location: Britsh Columbia

Join Date: Jan 2014
Posts: 4

basically I have a vial of mix strains of bacteria but a single specie. I wanted to know %proportion of each strain in a time period.

differentiate between strains by pcr-multiplex amplify 5 microsatellite loci of length from 100bp to ~400bp
each strain have different pattern of these fragment size.

right now I am culturing at each time point and and pick out 10 colonies each time.

But what i heard is that next gen dont really need culturing them into media agar plate.

Just looking for alternatives...with luck that it could help with finer resolution in %proportion
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