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Old 01-20-2014, 02:58 AM   #4
Location: Perth

Join Date: Sep 2012
Posts: 55

Hi Fadss, Yes NGS (primarily 454) has been used to type STR's. The main application to date has been the CODIS human ID markers used in forensics. We are yet to try it on our MiSeq but, it should work better than 454 and PGM due to the low homopolymer error.
check out:
Bornman, D., Hester, M., Schuetter, J., Kasoji, M., Minard-Smith, A., Barden, C., Nelson, S., Godbold, G., Baker, C. , Yang, B. 2011. Short-read, high-throughput sequencing technology for STR genotyping BioTechniques 2012.

Fordyce, S.L., Įvila-Arcos, M.C., Rockenbauer, E., Bųrsting, C., Frank-Hansen, R., Petersen, F.T., Willerslev, E., Hansen, A.J., Morling, N. , Gilbert, M.T.P. 2011. High-throughput sequencing of core STR loci for forensic genetic investigations using the Roche Genome Sequencer FLX platform Biotechniques 512: 127-133.
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