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Old 03-25-2010, 08:48 AM   #24
krobison
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Location: Boston area

Join Date: Nov 2007
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Just to note: another strategy that has been employed for this problem is to pool genomic DNA from multiple individuals, digest with a restriction enzyme, size select and make libraries from that defined fraction. I believe the recent chicken paper in Nature did this and definitely it has been published for other species (cows & pigs?).

By performing the restriction digestion & size selection you essentially create a smaller genome, which can then be sequenced exhaustively.
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