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Old 05-19-2015, 08:08 AM   #6
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
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Quote:
Originally Posted by Jfly7 View Post
Thanks for the reply.
I am doing genomic DNA library prep and am using HiSeq 2500 100bp PE reads. The reason I want libraries a bit longer than what I got is because a lot of fragments were short and therefore sequenced into the adapter, so we lost a fair amount of sequencing coverage. I would like to avoid this this time around.
Just use less Ampure during the clean up.
You will get a wide range of sizes using Nextera. Getting the insert sizes of amplicons that are sequenced to increase in length is just a matter of removing the shorter amplicons. (Shorter amplicons seem to cluster preferentially.)

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Phillip
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