View Single Post
Old 05-19-2015, 08:17 AM   #7
pmiguel
Senior Member
 
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,315
Default

Quote:
Originally Posted by NextGenSeq View Post
What I find most annoying about Nextera is the insertion bias.
Every Nextera I've ever seen shows unusual GC bias in the first 15 basepairs (see attached).
Nextera is Tn5. When you recruit a natural protein to do a job for you, you recruit its natural proclivities as well. Sure they have engineered it a little, but not enough to completely remove its insertion site preferences.

--
Phillip
pmiguel is offline   Reply With Quote