View Single Post
Old 05-22-2015, 08:48 AM   #10
Simone78
Senior Member
 
Location: Basel (Switzerland)

Join Date: Oct 2010
Posts: 205
Default

Interesting! When using our home-made Tn5 the tagmentation was actually INHIBITED by MnCl2 and worked only with MgCl2 (with 5 mM final being the optimal conc, while higher conc had a deleterious effect).
Thanks for sharing,
Simone
Simone78 is offline   Reply With Quote